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KMID : 0861020080230020099
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Korea Journal of Herbology
2008 Volume.23 No. 2 p.99 ~ p.109
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The Effects of Chelidonii Herba on the Proliferation of Eosinophils and Activation of Immuno-cells in Asthma-induced Mouse1)
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Roh Seong-Soo
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Abstract
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Objectives: The present study was to investigate the effect of extract of Chelidonii herba (ECH) on the proliferation and activation of eosinophils which were prepared from lung cells of asthma-induced mouse by ovalbumin (OVA) treatment.
Methods: C57BL/6 mouse was exposed to OVA three times a week for 6 weeks. The mouse lung tissues were dissected out, chopped and dossiciated with collagenase (1 ㎍/㎖). Eosinophils were activated by rIL-3/rmIL-5 co-treatments. The lung cells were treated with ECH, incubated for 48 hr at 37℃, and analyzed by flow cytometery, ELISA, RT-PCR, and immuno-histochemical analysis.
Results: In FACS analysis, number of granulocyte/lymphocyte, CD3e-/CCR3+, CD3e+/CD69+, CD4+ and CD23+/B220+ in asthma-induced lung cells were significantly decreased by ECH treatment compared to the control group. And mRNA expression for IL-4, IL-5, IL-13, CCR3 and eotaxin in asthma-induced lung cells, which was induced by rIL-3 plus rmIL-5 treatments, was significantly decreased by ECH treatment. In ELISA analysis, production levels of IL-3, IL-5, IL-13 and histamine in asthma-induced lung cells, which were induced by rIL-3 plus rmIL-5 co-treatment, were significantly decreased by ECH treatment. ECH treatments significantly inhibited the proliferation of eosinohils prepared from asthma-induced mouse lung tissues compared to the non-ECH treated control cells. Immunohistochemical analysis
revealed that ECH treatment significantly decreased the levels of eosipnphil activation compared to non-treated cells.
Conclusions: The present data suggested that Chelidonium majus L. may have an effect on the inhibition of parameters associated with asthma responses in eosinpophils, and thus implicate the possibility for the clinical application of Chelidonium majus L.
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KEYWORD
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OVA-induced asthma, Chelidonii herba, Eosinophil, CCR3, Eotaxin, Immunocytochemistry
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